Middle range protein MW marker - Names and Identifiers
Middle range protein MW marker - Physico-chemical Properties
Middle range protein MW marker - Introduction
The Middle range protein MW marker (Middle range protein MW marker) is a reference standard used to estimate the molecular weight of the protein to be tested. It is usually composed of proteins with known molecular weight and dyes, and is loaded on a SDS-PAGE gel for electrophoretic separation. By comparing the migration distance with the molecular weight of these standard proteins, the molecular weight of the protein to be tested can be roughly estimated.
Middle range protein MW marker has the following properties:
1. Wide molecular weight range: usually between 10-250 kDa, suitable for proteins in the medium molecular weight range.
2. Diversity: The standard contains a variety of molecular weight proteins, which can meet the needs of different experiments.
3. Easy to visualize: It exists in the form of dye, and its migration position can be visually observed by dyeing or other detection methods.
Middle range protein MW marker has a wide range of applications:
1. Determine the molecular weight of the unknown protein: By comparing the migration distance with the standard, the molecular weight of the protein to be tested can be estimated.
2. Verify protein purity: Used to verify the success of the protein purification step.
3. Evaluation of protein degradation and modification: By analyzing the migration pattern of proteins on the gel, we can initially understand the degradation and modification of proteins.
The method of preparing Middle range protein MW marker includes two steps:
1. Select a protein of known molecular weight: Prepare a standard by sampling a protein solution of known molecular weight.
2. add dye: mix protein of known molecular weight with dye (such as Bromophenol Blue or Coomassie Blue, etc.) to form a dyeing standard.
When operating the middle range markers of the Middle range protein MW marker, the following safety information needs to be noted:
1. pay attention to the operation specification: operate in accordance with the relevant provisions of the laboratory to avoid harm to people and the environment.
2. Avoid eating by mistake: The markers used in the experiment may contain toxic ingredients and should not be eaten by mistake.
3. Avoid contact with eyes and skin: markers and other experimental materials may be irritating to skin and eyes, and appropriate protective equipment should be worn during operation.
Last Update:2024-04-09 02:00:55